Gingerol and shogaol on red ginger rhizome (Zingiber officinale var. Rubrum) using high-performance liquid chromatography

Red ginger ( Zingiber o fficinale var. Rubrum) is one of the traditional medicines Zingiberaceae family, which contains phenolic ketone compounds, including gingerol and shogaol. At high temperatures, compounds become unstable will change into This study optimized conditions for simultaneous separation 6-gingerol, 8-gingerol, 10-gingerol 6-shogaol from red extract using High-Performance Liquid Chromatography method. The analytical consisted five different methods by modifying composition mobile phase, elution system, flow rate phase optimum UV wavelength. best a ratio water: acetonitrile in method 4 with gradient system 0 min (65:35); 1.5 (40:60); 5-6.5 (10:90); 7.5–9 (0:100); 9.5–12 was 1.1 mL/min at an wavelength 230 nm retention time 6-gingerol compound, 6-shogaol, 8-gingerol respectively were 4.947; 6.168; 6.554; 7.412 its resolution 2.267; 1.575; 1.315; 2.215. Then tailings factor asymmetry values obtained average value ± 1.